Hantavirus L protein exhibits shutoff activity mediated by its N-terminal endonuclease domain
B1 Crossref Works API · 2026-04-14 · United States
Abstract The hantavirus L protein is a viral polymerase essential for viral transcription and replication; however, its expression in mammalian cells has been notoriously difficult. In this study, we achieved robust plasmid-based expression of the L protein by combining a T7-driven system with mutations that reduce endonuclease activity. This strategy was pivotal, as conventional RNA polymerase II (Pol II)-dependent systems failed to yield detectable expression. Although wild-type L protein was barely detectable, its presence suppressed co-expressed genes
Read at source (Crossref Works API) →
HORIZON metadata
| Source | Crossref Works API (crossref) |
|---|---|
| NATO rating | B1 — see methodology |
| Country | United States |
| Reported date | 2026-04-14 |
| Ingested at | 2026-05-12 00:50 UTC |